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Because the use of Sedfit requires some theoretical background on sedimentation analysis, there are websites with introductions in 

    * Lamm equation solutions 

    * systematic noise analysis 

    * size-distribution analysis 

    * ls-g*(s) 

    * van Holde-Weischet analysis

    * Compressibility of water and organic solvents

    * Sedimentation in dynamic self-forming density gradients

    * c(s) based analysis of rapidly interacting systems

    * generating simulated data


There is also a step-by-step tutorial on how to use Sedfit.


Note also the Experimental Protocols website for how to plan, conduct, and plan analysis of AUC experiments.