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This website provides information on Sedfit, a software for the analysis of analytical ultracentrifugation and other hydrodynamic data, written at the National Institutes of Health and distributed without charge for research use. The program can be downloaded from here, and it is also available directly from sedfitsedphat.nibib.nih.gov, or it can be sent as an email attachment from the author on request). This website was written by the author in his private capacity, and no official support or endorsement of NIH is intended and should be inferred.
The main purpose of this website is to provide information for using the software, such as an online help reference information, and background information (tutorials) on direct boundary modeling, FAQs, references, examples and more. See the disclaimer.
A general introduction to the study of protein interactions by analytical ultracentrifugation can be found at the website of the DMA/LBPS at NIH. This includes an introduction to the general principles of AUC and detailed experimental protocols.
March 4 - 8, 2013: Thermodynamic and Hydrodynamic Analysis of Marcomolecules in Solution - learning SEDFIT and SEDPHAT at the Laboratorio Nacional de Biociencias, Campinas, Brazil
contact Dra. Ana Carolina Migliorini Figueira, Spectroscopy and Calorimetry Lab, LNBio, Campinas, SP, Brazil (firstname.lastname@example.org)
May 20 - 24, 2013: Biophysical Methods for the Thermodynamic Analysis of Macromolecular Interactions at the National Institutes of Health, Bethesda, Maryland, U.S.A.
by the Foundation of Advanced Education in the Sciences, NIH; for registration visit http://events.r20.constantcontact.com/register/event?oeidk=a07e6w99ayu0b7aa7de&llr=k4uamblab
Please join the Sedfit Users Group email list (reaches currently ~ 500 AUC users) for technical advice and discussions with other users on software issues and applications. You can also sign up to the SEDPHAT-L listserv for discussion of applications of SEDPHAT and questions of global analysis of ITC, AUC, DLS, SPR, and other biophysical techniques.
The main features of Sedfit include:
|analysis of data from: sedimentation velocity, dynamic light scattering, and sedimentation equilibrium. Sedimentation velocity data can be acquired using absorbance or interference optics, in conventional loading configuration or synthetic boundary, analytical zone centrifugation, and different cell geometries|
|continuous size-distributions c(s) with many variants of prior knowledge for sedimentation velocity analysis with maximum entropy regularization|
|size-and-shape distributions c(s,M)|
|Bayesian incorporation of prior for enhanced resolution of distributions|
|Lamm equation models for: discrete non-interacting species, self-associating systems (1-2, 1-3, 1-2-4, 1-4-8, 1-m-n), non-ideal sedimentation --> see extension for global analysis of interacting systems in Sedphat|
|apparent sedimentation coefficient distribution ls-g*(s) and van Holde-Weischet analysis G(s) (both for absorbance and interference data)|
|all sedimentation velocity models for direct boundary modeling with algebraic noise elimination|
|corrections for water compressibility (and compressible organic solvents)|
|continuous size-distribution models for dynamic light scattering and sedimentation equilibrium|
numerous statistical functions, loading options, and general purpose tools
extension to global analysis in Sedphat
Sedfit is closely related to Sedphat, which provides global modeling capability for both sedimentation equilibrium and sedimentation velocity data. It also can serve as a platform for the global analysis of a variety of isotherms from different biophysical disciplines.
|accounts for finite time of absorbance scanning|
|Extended multi-threading support for faster analysis on computers with multi-core processors|
|Bayesian prior knowledge for enhanced resolution of c(s) (links to paper and how to use in SEDFIT)|
|faster Lamm equation solutions (description)|
|new optimization tools: Marquardt-Levenberg and simulated annealing (how to use in SEDFIT)|
|real-time testing for attainment of sedimentation equilibrium (how to use in SEDFIT)|
|new user interface (new color scheme, wizard messages, graphical editing of data points and scans, dragging limits with the mouse, quick change of cells, pre-set limits for automatic integration)|
|version 9.4: new specialized c(s) models (wormlike chain, floating vbar), extended calculator, display, and other utility functions (improved 6-channel data support)|
|version 9.3: size-and-shape distributions c(s,f) and c(s,M), and the scale-relationship free general c(s,*)|
|sounds, serialization of analysis (apply the same analysis automatically to data from several cells)|
|version 9.2: optional speeding up of c(s) analyses by slight radial pre-averaging of scans, faster Lamm equation solutions with permeable bottom model, c(s) model with user-defined M-s relationship, optional updating of display while fiitting, restoring previous c(s) analyses, loading data via drag-and-drop, importing SEDPHAT xp-files, and other new and improved utility functions.|
|new tutorials on solvent compressibility, and self-forming density gradients|
|new Sedphat hybrid bimodal c(s)-discrete species model combining 2 c(s) distributions with discrete species, for example allowing to link molar mass into integral multiples|
|version 8.9: Export data to Sedphat, and spawn Sedphat automatically with current data (and c(s) hybrid model).|
|new in version 8.8 (03/04): maintenance update with a few bug fixes and added flexibility|
|new in version 8.7 (09/03): sedimentation in inhomogeneous solvents, like sedimenting co-solutes and compressible solvents, including corrections for water compressibility; extended Monte-Carlo error analysis for weight-average s-values or concentrations of trace components; differential second moment method for sw; faster c(s) fitting; improved ellipsoid shape calculator; bimodal c(s) with two f/f0 parameters, back-transform for g(s*), several new utilities and bug fixes|
|new mode for REALTIME analysis|
|Bayesian analysis of trace components (AAPS Journal 10 (2008) 481-493)|
|Bayesian prior knowledge (Biomacromolecules 8 (2007) 2011-2024)|
|new Lamm equation solutions (Computer Physics Communications in press)|
|size-and-shape distributions c(s,M) and scale-free general c(s,*) (Biophysical Journal 90 (2006) 4651-4661 full text )|
|interpreting c(s) for reacting systems: Biophysical Journal 89:651-666 and Biophysical Journal 89:619-634|
|tutorial review of analytical ultracentrifugation in protein science (Protein Science 11 (2002) 2067-2079)|
|new review of the strategies and numerical methods for sedimentation coefficient distributions in Methods in Enzymology (in press)|
|studies of strategies for using sedimentation velocity for protein self-association: Analytical Biochemistry 320:104-124|
|macromolecular sedimentation in the presence of sedimenting co-solutes (dynamic density gradients): Biophysical Chemistry 108:187-200|
|corrections for solvent compressibility Biophysical Chemistry 108:201-214|
A snapshot from the direct boundary modeling of data from a sample of IgG, analyzed with a c(s) distribution of Lamm equation solutions illustrates the rich information contained in sedimentation velocity data.
This example also illustrates some of the main ideas of Sedfit: loading data from the entire sedimentation process, use of systematic noise decomposition (and subtraction), modeling with finite element solutions of the Lamm equation. If we expand the scale of the continuous sedimentation distribution c(s) with maximum entropy regularization shown above, it can be seen that the c(s) analysis reveals the presence of several oligomeric species and a smaller species:
Similarly, the analysis of data from a BSA sample
shows the presence of dimer and trimer in the sedimentation coefficient distribution c(s)
Transformation to a molar mass distribution (assuming that all species have a similar frictional ratio) is consistent with the oligomeric BSA species:
Analyses free of scale-relation ship assumptions of similar frictional ratios are available, such as the two-dimensional size-and-shape distribution c(s,f), c(s,M), and the general c(s,*).
Sedfit offers several different options for utilizing different prior knowledge on the sample under study (such as the number of discrete species, a self-association model, etc.). More information on these examples can be found in the step-by-step tutorial (BSA) and the example for using Sedfit (IgG).
For questions please contact the Sedfit Users Group email list .
search SEDFIT and SEDPHAT websites
The information contained herein is provided as a service with the understanding that the author makes no warranties, either expressed or implied, concerning the accuracy, completeness, reliability, or suitability of the information.
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